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[Music] here are images of a control and drug treated cells stained with dappy in blue and alpha small in red in this tutorial we will learn to measure the intensity of alpha smart staining and compare between the control and the treated let us import the control image to be analyzed by drag and drop method next split the color channels by clicking on image color and split channels at this stage the images are converted into 8-bit images and splits into 3 different images the image here in the first one is from the blue channel dappy and the second image is from the red channel alpha smart staining we can disregard the image from the third channel in green invert the images individually for both the blue and the red channels by clicking on edit and invert [Music] next adjust the threshold of the images individually by selecting image edges and threshold use the slider to threshold the blue channel image until the red mask covers the signal [Music] similarly threshold the red channel i
