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okay good morning everyone welcome back to the third day I was going to make the joke about how the weather is like this year around that Iamp;#39;m about 24 hours too late the weather isnamp;#39;t like this youamp;#39;re on the weather isnamp;#39;t like the way it was yesterday youamp;#39;re the other so welcome again Iamp;#39;m going to talk about gene regulation with chief analysis and Iamp;#39;m told that that people are feeling very free to ask questions in the middle and I hope you will continue to do that anything you have have a question on and happy to answer it or at least attempt to answer it so weamp;#39;re going to talk about a few things here in general weamp;#39;re going to talk about transcription factor binding weamp;#39;re going to talk about what the challenges are in predicting transcription factor binding computationally at the end of the workshop you should be able to identify binding sites of known transcription factors and at the end of the lab that ve