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[Music] the overall goal of this procedure is to identify gram-negative organisms directly from blood culture broth using maldi-tof technology to do this five milliliters of a positive blood culture broth is transferred into a serum separating tube and centrifuged following the spin the bacteria containing fluid layer is transferred into a microcentrifuge tube and then centrifuged at low speed the supernatant is then transferred to a new microcentrifuge tube and centrifuged at high speed the resulting pellet is re-suspended in formic acid and analyzed by maldi-tof to identify the bacteria species present the main advantage of this technique over existing methods like traditional culture is the earlier identification of gram-negative bacteria the implications of this technique extend towards clinical microbiology because a diagnosis of bacteremia impacts on the choice of the antimicrobial prescribed by the clinician demonstrating this procedure will be leonardo bascoe whos one of the t
