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and yeah and the interest of speed here Im gonna stick with the pixel size of one for now okay so next then there are three parameters which have a relationship with the size that then you play theyre related to filters that were gonna apply to the image and were gonna do a trip into image a so we can actually show you how they operate in practice so these three are also its fairly important when it comes to improving your detection the first one is the background radius so during the detection shoot path will try and subtract a background value from every pixel on the image and then after that it will apply a threshold I should probably show you more about this threshold before we return to this so the threshold parameter is defined down here and if the threshold is high relative to the backgrounds the develop 0.1 Ill make it bigger then fewer pixels are going to pass that threshold and will detect fewer cells on the other hand if I make it a lot slower a lot lower so 0.01 Im p