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negative staining is a type of simple staining in this case the background is colored and the cell remains colorless in negative staining heat is not applied to fix the bacterial samples on the slide so bacteria are less distorted this is one method of negative staining your instructor may ask you to follow a different procedure but the end result is the same you will need two slides for this experiment place a small drop or loopful of sen at the end of each slide for cultures on solid media add a loop full of distilled water and emulsify a small amount of the culture in the sand water drop flame the loop get a loop full of water and add it to the drop of Sun you flame your loop remove the cap from the tube containing the solid culture pass the tube through the flame obtain a loop full of bacteria and mix it with the sand water drop on the slide you using the end edge of another slide spread out the drop to produce a smear varying from opaque black to gray the angle of the spreading s