Modify stain in PAP

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Aug 6th, 2022
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How to modify stain in PAP

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hi everyone for todayamp;#39;s topic we will be discussing about the papa nicolao staining technique but before that i want you to know that papa nicolau stain or the pop staining method is under the big umbrella of diagnostic cytology under diagnostic cytology it also belongs under exfoliative cytology so letamp;#39;s differentiate first what diagnostic cytology and exfoliative cytology means diagnostic cytology class simply means microscopic examination of cells from different body sites for diagnostic purposes diagnostic cytology includes exfoliative abrasive and aspirational but for today letamp;#39;s focus on exfoliative cytology deals with the microscopic study of cells that have been disqualmated from epithelial surfaces exfoliated cells may be found in smears that have spontaneously been shed or physically removed from epithelial and mucous membranes such as the vagina bucal mucosa and from body fluids such as the sputum urine plural peritoneal or acetic fluid the gastric ju

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The principle of pap stain Solution 1 has Hematoxylin stain, a basic nuclear stain. Solution 2 has OG-6, an acidic cytoplasmic stain, has orange G with phosphotungstic acid (6 denotes the used concentration of phosphotungstic acid). Orange G is an acid dye and stains keratin with a bright intense orange.
The pap stain has 3 solutions having 6 dyes. Solution 1 has Hematoxylin stain, a basic nuclear stain. Solution 2 has OG-6, an acidic cytoplasmic stain, has orange G with phosphotungstic acid (6 denotes the used concentration of phosphotungstic acid).
Wash slides in deionized or distilled water for 5 min at room temperature. Destain slides in 0.5% HCl in 70% ethanol for 5-15 min at room temperature. Wash slides in deionized or distilled water for 5 min at room temperature. Air dry sections.
Composition of the Reagents of Papanicolaou Staining (Pap stain) Hematoxylin = 2.5g. Ethanol = 25ml. Potassium alum = 50g. Distilled water (50C) = 500ml. Mercuric oxide = 1-3g. Glacial acetic acid = 20ml.
Results: The study showed that the modified Papanicolaou method took three minutes and the standard Papanicolaou method took 20 minutes to stain each bunch of slides.
Special stains are used as per requirements: Modified Ziehl Neelson (for acid fast bacilli), Gram staining (Bacteria), Mucicarmine (mucins), PAS (for glycogen, fungal wall, lipofuscin, etc), Oil red O (lipids), Perls Prussian blue (iron), modified Fouchets test (bilirubin), etc.
The Papanicolaou stain is undoubtedly the most commonly used in cytology. It has three cytoplasmic dyes, orange G, eosin Y, and light blue. It can stain cells blue or blue-green (basophilia, cyanophilia), pink (acidophilia, eosinophilia), orange (orangeophilia), or indeterminate (gray-blue).
Types of Staining Based on the process, staining is grouped into three types: Simple staining, Differential staining, and Special staining. Differential staining involves multiple stains to distinguish between different types of microorganisms or structures within a specimen.

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