Link stain in PAP smoothly

Aug 6th, 2022
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DocHub is a world-known online document editor trusted by millions. It can fulfill almost any user’s request and meets all necessary security and compliance standards to ensure your data is well protected while modifying your PAP file. Considering its powerful and intuitive interface offered at an affordable price, DocHub is one of the best choices out there for enhanced document management.

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How to Link stain in PAP

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the fixation done between the 15 to 20 minutes of session we want to go to the next step which is the staining process okay before that we have to transfer from the fixed 95 alcohol slide into this slide holder [Music] right [Music] so please make sure that your slide will not dry up completely so you have to make it as soon as fast as possible okay so next we transfer this to the first [Music] weaker which is we transfer to another all right after 40 seconds we go to the third step which is the running tap water [Music] so please make sure the tap water is one running [Music] that means that for leave it for 40 seconds all right after 45 seconds done let me do the hot that which is hematology so we leave it for around 45 seconds okay after 45 second done we go to the next step which is the running tap for 40 seconds alright it for 40 seconds okay after 40 seconds the next step is we dip it in acid alcohol so this is one of the critical parts in the cleaning which if you over dip it it

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The principle of pap stain Solution 1 has Hematoxylin stain, a basic nuclear stain. Solution 2 has OG-6, an acidic cytoplasmic stain, has orange G with phosphotungstic acid (6 denotes the used concentration of phosphotungstic acid). Orange G is an acid dye and stains keratin with a bright intense orange.
[2] The Pap stain has been most frequently used for screening of cervical cancer.
Papanicolaou stain (also Papanicolaous stain and Pap stain) is a multichromatic (multicolored) cytological staining technique developed by George Papanicolaou in 1942. The Papanicolaou stain is one of the most widely used stains in cytology, where it is used to aid pathologists in making a diagnosis.
Wash slides in deionized or distilled water for 5 min at room temperature. Destain slides in 0.5% HCl in 70% ethanol for 5-15 min at room temperature. Wash slides in deionized or distilled water for 5 min at room temperature.
The conventional Pap stain has 24 solutions, of which three are acid dyes, six water, one is chemically defined bluing agent, eight 95% ethanol, three absolute alcohol and three xylene Gill, 2006.
The pap stain has 3 solutions having 6 dyes. Solution 1 has Hematoxylin stain, a basic nuclear stain. Solution 2 has OG-6, an acidic cytoplasmic stain, has orange G with phosphotungstic acid (6 denotes the used concentration of phosphotungstic acid).
The PAP stain is a polychromatic counterstaining method consisting of stains such as Orange G 6 (OG6) and modified eosin azure (EA). The strength of the Pap stain is such that it results in: Well-stained nuclear chromatin.
The Papanicolaou (PAP) Stain Kit (Cytology Stain) is designed to differentiate between a variety of cells in vaginal smears for detection of vaginal, uterine and cervical cancer. In addition, this procedure is valuable for staining a variety of other bodily secretions and cell smears.

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