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[Music] okay so the last trichrome stain that were going to troubleshoot is the MSB stain if we begin with this slide here at the moment this actually shows the outcome that we expect to see so we have good strong defined nuclear features theres quite a variety of cell morphologies in this section of placenta but in any case those nuclei are really nicely demonstrated the fibrin being demonstrated by this red staining the scarlet red is nicely demonstrated if we scan around we can see other areas of fiber in there as well and the collagen has stained up really nicely as well the other thing that we can notice is with respect to the red blood cells the majority of the red blood cells clearly stained a yellow color and that indicates that the slide has been post fixed in the picric acid for the best part of an hour you know at least 45 minutes to an hour to achieve that nice even density and so yeah this is a really nice slide I think Ive given that about a seven and so thats what yo