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hi everyone welcome to tutorial 47 of our introductory Python for image processing tutorial series in this tutorial let's talk about PI stack reg library in Python that's an amazing library for image registration now in the previous tutorial I talked about 3 or 4 other ways here is an including optical flow and of those optical flow is something that I definitely recommend exploring but PI stack Rajee is a very good library for microscopy applications especially whether it is FIPS 'm alignment or other light microscope alignment it has a lot of functions that allows you to perform sub pixel registration in many ways and many ways the includes rigidbody for example f-fine and scaled rotation and so on so I did provide a quick overview of this in my previous tutorial but not much to look at here let's actually explore this in spider but one thing I should again mention is this is pretty much the same algorithm that's available under image J as turbo arrays or stack arrays and it's writt...