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Tutorial 47 discusses the PI stack reg library in Python, which is great for image registration, especially in microscopy applications such as FIPS 'm alignment. The library allows for sub-pixel registration with functions like rigidbody, affine, and scaled rotation. It is similar to the algorithm in image J as turbo arrays or stack arrays. The previous tutorial explored other methods like optical flow, but PI stack reg is highly recommended. Let's explore this further in Spider.