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this video explores the link between the signals and biological samples and the digital data from microscope cameras understanding this relationship can help you set the ideal image acquisition conditions to achieve the highest quality images and data first excited fluorophore or fluorescent protein labels on the target emit fluorescence light the light intensity increases when more targets are in the sample or when we apply stronger excitation light then the fluorescence light enters the camera sensor through the microscope optics and stimulates the photoelectrons in the sensor stronger light stimulates more photoelectrons the photoelectrons convert into a digital signal which is proportional to the incident light intensity the detected signal contains actual signal and background signal the background signal also known as background noise can vary your actual signal if there is no background signal you can identify any weak signals to detect your target you need a high enough ratio...