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hello friends welcome to bmh learning this video is about cross-linking mass spectrometry cross-linking mass spectrometry analyzes protein protein interactions to better understand how proteins affect biological processes such as signaling cascades gene upregulation and energy production crosslinking for protein protein interaction studies enable visualization of the interacting regions further excel ms can be used in parallel with high resolution techniques such as cryo-electron microscopy or x-ray crystallography to obtain structural information cross-linking experiments provide distance constraints between regions within the protein creating three-dimensional structure information a general topology of the protein structure for protein complexes excel ms can also be used to determine organization of protein complexes general mass spectrometry workflow the diagram is showing a typical chemical cross-linking mass spectrometry workflow for characterizing protein protein interactions a
