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The following content is provided under a Creative Commons license. Your support will help MIT OpenCourseWare continue to offer high-quality educational resources for free. To make a donation or view additional materials from hundreds of MIT courses, visit MIT OpenCourseWare at ocw.mit.edu. ELIZABETH NOLAN: What were going to do today is just discuss a few aspects of cross linking. So we decided it was important to introduce this within recitations this year, because cross-linking comes up time and time again. And theres different ways to do this, and different strengths and limitations to different approaches. So I guess in just thinking about this, what is cross-linking? So if you say, oh, Im going to use a cross-linker for my experiment, what does that mean? AUDIENCE: Forming a covalent linkage between two molecules of study. ELIZABETH NOLAN: Yeah. So theres going to be formation of some sort of covalent linkage between two or maybe more-- right? Because some cross-linkers can h