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[Music] once our pathologist accepts the sample for testing the dna or rna from the sample is extracted the sample undergoes a simple enzyme digestion to facilitate the extraction of nucleic acids from the tumor cells the dna and rna is purified and the amount is quantitated by means of a fluorescent assay this ensures that enough unique molecules have been extracted from the patients sample to continue with the sequencing process and yield robust reliable results on the foundation 1 or foundation 1 heme assays during library construction fifty to a thousand nanograms of dna is broken into small fragments in a randomized manner through sonication this process creates rich complex and unique dna and rna fragments that are representative of the patients tumor sample it is this complexity which drives the depth of coverage during sequencing and increases the chances of capturing relevant alterations during computational analysis magnetic beads are added to the sample and the mixture is
