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The tutorial introduces the concept of linkage, which involves genes closely located on a chromosome. Linked genes do not assort independently. A test cross with A and B genes in heterozygotes shows non-linkage. The expected genotypic frequencies are tested using the chi-square test, with observed frequencies of 20, 26, 30, and 22 instead of the expected 25. The chi-square value for the comparison is 1.76.
