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hi im jennifer waters the director of the nikon imaging center at harvard medical school and today is a quick presentation on a very important property of lenses numerical aperture numerical aperture is critical because it limits both resolution and the brightness of your image in light microscopy we define resolution as the distance by which two objects must be separated in order to distinguish them as separate from one another in the image of the objects generated by the microscope and in epifluorescence microscopy according to the rayleigh criterion that distance is equal to a constant 0.61 times the wavelength of light that is generating the image so the emission wavelength of light in fluorescence divided by this property of the lens called numerical aperture which we abbreviate as n a numerical aperture is marked on every objective lens so right after the magnification youll see a slash and then a number and that number is the numerical aperture of the lens the numerical apert