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Aladin creations [Music] sir what is you doing Im going to perform one of specialists a call combined alcian blue and pas staining technique what is combined alcian blue and pas staining technique actually the staining procedure is combination of alcian blue and periodic acid shift stain but why this technique this technique is important to demonstrate presence of mucin and differentiate acid mucin from neutral musin great but how first perform alcian blue stain ampersand then performed periodic acid shift method then so while the acid musin in tissue stained with alcian blue and get blue color those remaining acidic machines which are also positive will be chemically blocked and will not react further during the technique after neutral musin stained with periodic acid shift stain and magenta color so we can clearly differentiate acid mucin from neutral music but if tissue present glycogen it also stained with periodic acid shift stain and neutral mucin yes you are correct so dyes tas