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foreign to split the channel colors of an image open the image in the image J software in the image J toolbar click on image color and split channels at this time the image has been split into three different channels the blue channel the green Channel and the red Channel colors if the image output is a grayscale the image color can be applied again click on image look up tables and select the color blue for the dappy staining similarly the other channel colors can be applied to the remaining images once completed these images can be saved in any format of your choice to save the image click file save as Tiff and Save the other channel color images can also be saved in a similar way scale bars can also be added before saving these images please visit the tutorial video on how to add scale bar using image J the link to this video is in the description below to merge images of different Channel colors open all the images using the image J software if you would like to modify the image co