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this Eliza tutorial series is kindly sponsored by cow biotech please click the link below or in the video description to see what Eliza kits they have to offer today weamp;#39;re going to talk about how to prepare the Eliza plate for samples in order to really understand whatamp;#39;s going on here hereamp;#39;s a little schematic uh youamp;#39;re going to start with an empty plate which is special because it binds proteins with High affinity and so the first thing youamp;#39;re going to do is add a solution of capture antibodies to the empty Wells of a 96v plate over time those antibodies are going to adhere to the surface of the plate as shown here after coating the liiza plate with the capture antibodies thereamp;#39;s still going to be regions in the surface of the Elya plate that can bind protein basically the empty spaces between the capture antibodies and so the next step is going to be blocking the plate which essentially is trying to cover up any remaining available spac