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you think that with a 96 ball plate youamp;#39;d be able to test well 96 things right unfortunately you have to account for Edge effects as well as your controls effects is basically where the outer edges the outer Wells of your plate experience different conditions than the inner Wells especially with regards to their temperature and their volume because of evaporation so itamp;#39;s worse than like the outer corners and then the outer rim and then as you go in it gets gradually better so basically what happens is that in your dish these outer Wells are going to experience faster and more evaporation and theyamp;#39;re going to experience like a higher temperature or at least adaptation to the temperature so for example if you were to stick your plate in the incubator those outer Wells so is you stick your like the 37 plate degree incubator after like preparing your plate at room temperature well now the outer Wells are going to get hotter first if you are like cell plating cells a
