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hereamp;#39;s an example of that photo bleaching that I mentioned in this gallery you can see that in the bottom row is just PBS with glycerol and this dye which is a forcing or fitzy phalloidin photo bleaches away and becomes done very quickly whereas if you use prolong gold or even better a prolonged diamond you see that you retain your signal over that entire minute now I mean it doesnamp;#39;t sound like a lot of time but it actually itamp;#39;s quite long for imaging and you need time in order to orient yourselves and and find what you need without the dye just disappearing so choosing an anti fade is important but what else can you do well you want to reduce the light intensity and the duration that itamp;#39;s on the sample because the more light there is the stronger the light the longer the life and more photo bleaching your hats so you want to be quick about imaging about setting it up if you use a traditional microscope you might even throw in a neutral density f
