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in all eukaryotes the peripheral endoplasmic reticulum is a continuous network of membrane tubules and cisternae that emerges from the nuclear envelope in budding yeast fluorescence microscopy suggests that the bulk of the er forms a tubular network at the cell cortex which is connected to the nuclear envelope by a handful of er tubules traversing the cytoplasm gear vaults from the university of colorado boulder decided to take a higher resolution look at yeast er structure using electron tomography we take e-cells and we high-pressure freeze them so theyamp;#39;re frozen rapidly and so that preserves the structures in as natural state as we can and then we slice them into 150 to 200 nanometer six sections and this was work done by matt west and nesha necessary in my lab where for each section they would trace every membrane-bound structure and then this imod program reconstitutes all the layers back together to redraw the continuous layers of membrane and we defined what was er as wh
