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Welcome to mastering and guidelines in ultrasound and Echo The most common and reliable technique for measuring ejection fraction is Simpson or disk method. in this technique we just go get apical four ( and two) chamber view. correct apical four chamber view and then zoom on the left ventricular and then we go and trace endocardium in two spots: end diastolic and end systolic in apical four and apical two chamber views There is a general rule for tracing endocardium: we donamp;#39;t include papillary muscle and trabeculation or Trabecula. as you see most of the time we have trabeculation more prominent in the right ventricular But sometime in the left, as you will see in this image trabeculation as round smooth cylinder or irregular bundle muscle that cover endocardium. On these two cases if we needed to trace endocardium, for example measuring left ventricle mass We should trace endocardium too and again, we donamp;#39;t include Trabeculae and papillary muscle and we just traced co