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friends today we discuss about the straining techniques we know the live bacteria do not show much structural detail under the light microscope due to the lack of contrast hence it is very customary to use training techniques to produce a color contrast the bacteria may be stained in the living state or by killing them routinely we use methods of straining of bacteria that involve drying and fixing the smear which kill the bacteria okay then uh the protoplasm of the bacteria have an Astic nature so the bacteria always have an affinity to basic ties the staining techniques that commonly used in bacteriology are simple stains negative staining impregnation methods and differential stains and simple stains a we use a methylene blow or a basic Fusion they provide a color contrast but they embed same color to all the bacteria so that we cannot differentiate between various types of bacteria and a negative staining uh we use enthane ink or nigrosin that are emulsifier with the sample or orga
