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okay so weamp;#39;re going to do a spot test and before you do your spot test uh whenever you put the bacteria actually on the plate you labeled your plate um and so iamp;#39;m going to be doing three different samples and then also a negative control um so testing through different phases and then the negative control and your negative control will be phage buffer if you have spots in that area then your test wasnamp;#39;t um done correctly youamp;#39;re gonna make sure youamp;#39;re pipetting your uh phage into the correct area on your spot plate and itamp;#39;s obviously reversed whenever you put it down but just remember where you put your labels to do a spot test youamp;#39;re just going to take three microliters of your phage sample and pipette it on the plate and make sure to not touch the plate whenever you pipette it but you do want to get low to the plate so you donamp;#39;t splash the page in other areas of the plate and then whenever you finish putting your spots on