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Intro Music: Analytical Methods in Geosciences Inserting Samples Into SEM To insert your sample in the SEM, first make sure its not on standby by pushing the button. Then go to the computer and push the button on the computer which allows the SEM to open the front door. You have to wait until the indicator on the chamber goes from being red to green. Like this. Now I can open the sample chamber by pushing up and let go. Ill do it by itself the rest of the way. You insert your sample into the sample chamber. And then insert the sample chamber back into the instrument. Push upwards then let go to close the door The machine will then pump down through a vacuum automatically. You can see your sample there. Credits.