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Hello, this is Jhoynner Martinez with the Canadian Centre for Electron Microscopy. The instructor in the video is Chris Butcher, also with the Canadian Centre for Electron Microscopy and today you will be learning how to vent and insert your sample in the chamber. Our microscopes are always kept under vacuum, so its always good to make sure that they are. The first thing that we you tend to do, is well we want to open the chamber, so were going to vent it, okay. You can also vent it using the system in front of the actual SEM. Usually this takes about 30 seconds and then our window will pop up. On the left side you will see the different size of holders that the software has available. Obviously we dont have all of them, so pick accordingly. And also you will see the protruding height in orange, okay. This is very important that you do measure the protruding height from the top of the side of the holder to the highest point on your sample. Opening the chamber ,okay, you just quic