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Heroes of basic run through of differential expression and plotting volcano plots heat Maps gsea PCA and it was all in Python without r so weamp;#39;re going to be doing bulk differential expression using pi D seek 2 which is a re-implementation of dc2 which is normally run in R and python instead so we can do this whole analysis and our Jupiter notebook you can by dc2 like this and weamp;#39;re going to go ahead and import the modules from it that we need as well as pandas or some data frame manipulation I already have accounts CSV so at this step weamp;#39;re assuming you have account CSV or any format of accounts table Iamp;#39;m just going to go ahead and read mine in if itamp;#39;s not a CSV youamp;#39;ll have to change how you import it here but thatamp;#39;s a pretty straightforward step so we have our accounts table with our Ensemble IDs and the samples we have to make a few changes first we want to set the Ensemble IDs to the actual data frame index column so ju