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welcome back guys a few days ago we talked about how to create an roi in fsl using fsl maths specifically we created a sphere around a series of x y and z coordinates you can do a similar thing in aphny using either 3d calc or even easier a program called 3d on dump so to show you how to do this first of all weamp;#39;re going to open up apni and here is an anatomical data set that iamp;#39;m going to use this is an m i space and letamp;#39;s say that i want to create an roi and the letamp;#39;s say right nucleus accumbens so iamp;#39;m going to right click here go to atlas location scroll down until i see right nucleus accumbens here we go okay so this is for another tutorial iamp;#39;m not going to get too much into it right now but notice that right is in the negative direction it has to do with the fact that weamp;#39;re in rai coordinates okay this is the default right now you can change that but just note down these numbers right here negative 12 negative eight negative ei