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this is a short video illustrating a technique we used to inject ranibizumab into the eye at the University of Iowa we first place a topical anesthetic into the eye and this is followed by a topical antibiotic next we inject a small amount of xylocaine subconjunctival ii usually this is in the superior temporal quadrant we raise a blob of the anesthetic and allow this to remain in place for three minutes or more for anesthesia while the anesthesia is taking effect we paint the area of the conjunctiva where weamp;#39;re going to inject with 5% povidone-iodine we then remove the ranibizumab from the bottle using the large bore syringe provided by the company this large syringe is then replaced with a 30 gauge needle and the syringe is checked to make sure we then have a point zero five milliliter or five milligram dose a lidded speculum is inserted into the eye an area three point five millimeters from the limbus is marked with calipers often will replace 5% povidone-iodine prior to inj