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this video will show you how to process your bio films it will cover three main steps first removing the planktonic cells these are the cells that are not a part of the bio film second staining the cells that remain in the bio film third solubilizing the cells so that we can accurately quantify the number of cells in each bio film first look at your plate youamp;#39;re checking for contamination if any of the blank walls are cloudy record these in your notebook next with water running you will shake the planktonic cells into the drain next fill three containers with distilled water these will be used to wash out any excess planktonic cells next plunge the plate into the first container and shake the cells out into the drain continue with the second and third containers now youamp;#39;ll fill one of the boats with crystal violet this will be used to stain the cells in the biofilm next set your multi-channel pipette to 100 microliters pick up eight tips and transfer 100 microliters of
