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my name is Laura Johnston Iamp;#39;m the scientific associate director at the flow core at U Chicago this video is the first in a series that will go over the practical steps of how to perform an actual flow cytometry experiment these videos are intended for users who already have some knowledge of flow cytometry we do have a flow basics course that is required to use our facility at U Chicago so really this is a follow up to that original course my goal here is to break down the practical steps of running a flow cytometry experiment and help you understand how each of these steps could ultimately affect your final results so in this first video iamp;#39;m going to go over the basic staining protocol and then the second video iamp;#39;m gonna build on this first video and discuss how to optimize the protocol the third video will go over a panel design fourth video weamp;#39;ll talk about experimental design which is mainly choosing controls and then the fifth video Iamp;#39;m goin
