Inject stain in NEIS

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Aug 6th, 2022
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How to inject stain in NEIS

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angioplasty is a procedure to open narrowed or blocked arteries caused by deposits of plaque if the blockage is not major the problem may be corrected by inflating the balloon several times to compact the plaque against the arterial wall widening the passage for the blood to flow through typically a device called a stent is placed within the coronary artery to keep the vessel open

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Mitochondria in the cell can be visualized by employing, 1) Altmanns stain- ing method or Heidenhains iron hematoxylin stain on fixed preparations, 2) the supravital staining method using Janus green, and recently 3) the supravital observation by means of the phase contrast microscope.
The NEISSER staining kit (diphtheria detection) consists of a combination of three NEISSER solutions: Methylene Blue, Crystal Violet and Chrysoidin. It is used in microbiology and bacteriology to detect Gram-positive pleomorphic rods such as Corynebacterium diphtheriae, the causative agent of diphtheria.
Neisser stain kits help identify filamentous bacteria by staining polyphosphates a gray to blue/purple color (Neisser positive). Positive color can be found in the cell membrane or in grannules within cells. Neisser negative bacteria stain brown.
In histology, histopathology, and clinical pathology, Perls Prussian blue is a commonly used method to detect the presence of iron in tissue or cell samples. Perls Prussian Blue derives its name from the German pathologist Max Perls (18431881), who described the technique in 1867.
The special suitability of this kit lies in the Neisser stain, which allows differentiation of morphological characteristics and granules in Corynebacterium diphtheriae. The staining assists in the identification and diagnosis of diphtheria infections.
The Neisser stain is a microbiological stain for visualising the polar bodies in the cytoplasm of gram-positive bacteria. This staining is used to identify diphtheria bacteria. Cells are stained with methylene blue and crystal violet in acidic solution and then counterstained with chrysoidin solution.

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