Inject stain in HWP

Use this walkthrough to inject stain in HWP in minutes

HWP may not always be the simplest with which to work. Even though many editing tools are out there, not all provide a easy tool. We created DocHub to make editing effortless, no matter the file format. With DocHub, you can quickly and effortlessly inject stain in HWP. On top of that, DocHub gives a range of other functionality including document creation, automation and management, sector-compliant eSignature services, and integrations.

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To inject stain in HWP, follow these steps:

Click Sign In or register a free account.
When directed to your Dashboard, hit the Add New button and choose how you want to import your file.
Use our advanced tools that can help you enhance your document's text and layout.
Choose the ability to inject stain in HWP from the toolbar and use it on document.
Review your text once more to ensure it has no mistakes or typos.
Click DONE to complete working on your document.

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How to inject stain in HWP

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What is the purpose of the HE stain?

H and E staining helps identify different types of cells and tissues and provides important information about the pattern, shape, and structure of cells in a tissue sample. It is used to help diagnose diseases, such as cancer. Also called hematoxylin and eosin staining.

How to destain HE slides?

Clinically, we de-stain HE slides as a regular protocol using 1:1 Xylene/Acetone solution to remove the coverslip and 1% Acid (HCl) Alcohol to remove the stains. Acid alcohol is used in regressive hematoxylin staining regularly and eosin is quickly removed by both acetone and alcohol.

What does eosin stain in the cell?

Eosin shows the cytoplasm, collagen, docHub tissue, and other structures that surround and support the cell as an orange-pink-red color.

What is the protocol for HE staining?

Protocol for HE staining: Place a drop of Permount on the slide using a glass rod, taking care to leave no bubbles. Angle the coverslip and let fall gently onto the slide. Allow the Permount to spread beneath the coverslip, covering all the tissue. Dry overnight in the hood.

Which step in HE staining is for deparaffinization?

Many HE and special stain protocols begin with step #1 as simply Deparaffinize and hydrate to water. In fact, nothing could be more complicated. The sections to be stained are surrounded by a matrix of paraffin wax, which is insoluble in water. All of the paraffin must be removed to ensure optimal staining.

Why is there a need to perform deparaffinization before the staining process?

As the paraffin wax is insoluble in water, it is necessary to remove it from the tissue section before applying any aqueous or water-based dye solution, to allow the tissue to successfully interact with the stain.

What is the HE differentiation step?

Differentiation is a procedural staining step related to a regressive HE staining method. When staining with hematoxylin, hydrochloric acid (for rapid differentiation) and acetic acid (for slower, more controlled differentiation) are the most commonly used components of differentiating solutions.

What is the bluing step in HE stain?

One of the steps in the HE procedure is bluing. As the name implies, this step converts the initial soluble red color of the hematoxylin within the nucleus to an insoluble blue color. The alkaline pH of the bluing solution causes the mordant dye-lake to reform in the tissue and become more permanent.

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