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applying a simple stain to a bacterial culture is a technique that is used for examining the size shape and arrangement of a specimen it uses a dye to stain the cells making them easier to see under a microscope in this experiment we will demonstrate the steps for applying a simple stain to the bacterial organism Staphylococcus start with a clean microscope slide use a china marking pencil to label one end of the slide with a specimen name draw a circle in the center of the slide because weamp;#39;re using Staphylococcus from a solid source weamp;#39;ll draw a circle about the size of a nickel turn the slide over so that the circle is on the bottom this will prevent the marking from mixing in with the sample add a drop of distilled water to the slide sterilize an inoculating loop by passing it through the flame of a Bunsen burner when the loop is cool touch the bacterial colony and transfer the bacteria to the slide use the loop to mix the cells and the water spread the mixture to co