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cleaning the syringe is very important 50% of Sedo nitrile kimwipe wipe the outside of the needle draw 50% of sino nitrile up into the syringe all the way to the top Iamp;#39;m at the 50 micro liter mark rejected to the sink repeat wipe pull up sovereign eject this syringe should now be clean when washing the loop this levers up this is down levers up when youamp;#39;re washing the loop Iamp;#39;m taking 40 microliters injecting the loop is here Iamp;#39;m putting excess solvent into the loop the excess will appear here on the other side the excess excess solvent will appear here in this overflow vessel so I do this twice if Iamp;#39;m injecting the same sample as I used before I donamp;#39;t really need to rinse the loop but if someone else has used the machine before me I want to flush out that compound from the injection loop when injecting actual sample I have almost the same procedure as flushing the loop I pulled my sample up to 40 microliters I see a air bubble here at the
