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for the injection we use a syringe this is the syringe this is the state of loading and this is the state injector we want to load you you switch this to this position okay for example switch it here yeah so now you can take your syringe and you can inject i try to inject only the methanol just to show you the example so you push then you switch it to the injection and you can see here so when you meet the injection you will find this peak this is the pressure this is the line of pressure and this one and this one is of the wavelet that you used so as you can see here the pressure gets down then go back to his place so injected only methanol there is nothing so we are just washing the carbon so here we have the two wavelengths that we are using 210 and 255 the maximum absorbance we have at 255 this is the solvent a solvent b the bottles are filled so this is the methanol and here we have the water the hpc3 you need to cover all with your bottles by the aluminum foil right so as
