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hello this is scott Kephart with quasar instruments and today iamp;#39;m going to talk about prepard of HPLC injection volumes to start out Iamp;#39;m going to draw a typical two position six port injection valve over here and do my other videos I go how to plumb this this type of injection port up but in this one weamp;#39;re just gonna be talking about the appropriate injection volume amount so what Iamp;#39;m doing is drawing the sample loop here in this case this will be from your injector this will be waste this will be from your pump and this will be to your column so what weamp;#39;re going to do is really look at the injection loop so Iamp;#39;m gonna draw that injection loop out as a straight tube and then Iamp;#39;m gonna draw vertical lines to represent the injection volume so there are the total injection amount so this is actually your injection loop and right here and right here is where your injection loop is plugging into the valve now when you load the sample on
