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so Iamp;#39;m about ready to inject my sample right now everythingamp;#39;s quiet says idle says standby I need to turn on the pump turn device on I hear the pump going itamp;#39;s idle meaning we havenamp;#39;t started our method yet but itamp;#39;s ready for me to do an injection for the injection Iamp;#39;ll follow the syringe protocols here everything looks good I have a pressure of a hundred and thirty three bars thatamp;#39;s about 133 atmospheres a lamp is on my solvent mixture is what it should be I will click here balance which is like baselining this part of the spectrum here all right so I baselined my UV detector and now it is ready for me to actually start my run inject my sample Iamp;#39;m flipping the switch now to start I will flip this to inject I will now flip the switch goes to run the timer started at zero minutes Iamp;#39;m now looking at retention time in the column here I can see Iamp;#39;m at point two three minutes here I can see my percent acetyl nit