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for this video we will demonstrate proper technique for manual gas chromatography injections to ensure reproducible injection volumes and retention times make sure that you are wearing the appropriate personal protective equipment when working in the lab first rinse the syringe with an appropriate solvent to ensure that itamp;#39;s clean here we will draw up six ten microliters volumes of acetone into a ten microliters syringe and expel the acetone washes into a waste beaker next weamp;#39;ll drop several volumes of air and depress the plunger quickly to remove residual wash solvent following this it is important to rinse the syringe with the sample several times first join the plunger up slowly and expelling to 10 micro liter sample washes into a waste beaker this rinses the syringe with the sample and flushes out any remaining solvent from the washing step we will rinse the syringe six more times with our sample now we will draw up one sample slowly to avoid air bubbles Injection t
