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hi iamp;#39;m mark from teledone isco and today weamp;#39;re going to show you how to run the acuprep hp 150 with the auto injector what you can see here is the accu-prep peak track software uh the graph at the top represents uh absorption units on the left for your uv signal and on the right is the the percentage b gradient as you can see weamp;#39;ve already got a gradient set up from 10 to 100 b down in the left hand corner is where you would set the parameters for your run so you would choose the appropriate size column which is set up in your your method and in tools there is a flow rate associated with the column and you select the solvents that are required for your purification we give this sample a name and then weamp;#39;re ready to run the purification in the bottom right hand corner you can also choose whether you want to collect all or adjust the peaks as detected by the system so if we hit play it will then give us our sample injection options as you can see itamp;#3