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traffic light I say looks at co-localization between red and green becomes yellow and then we are also interested in something that is only red it doesnamp;#39;t co-localize with green for instance and so by Counting this dot we get a sense of how much auto fetch is happening in ourselves itamp;#39;s one of the universally used as is itamp;#39;s used also using high content screening and so on and so forth and so it becomes a bread and butter for many auto fragile apps that are looking at autophagy flux for instance and the drawback always being that who wouldnamp;#39;t sit sit and count those dots you know and in our lab itamp;#39;s always been a young enthusiastic passionate kids who come in and we put them to task doing this and very quickly they learn that this is a very boring mundane thing and so we have to find new people we started working with appeal and we had a really interesting set of teams where they understood what these dots mean itamp;#39;s not a
