Inject identification in MBP

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Aug 6th, 2022

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How to inject identification in MBP

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Got questions?

Below are some common questions from our customers that may provide you with the answer you're looking for. If you can't find an answer to your question, please don't hesitate to reach out to us.

What is the isoelectric point of meat protein?

The isoelectric point of meat protein is around pH 5.0, and WHC is the lowest at that point. Generally, the WHC of meat proportionally increases with increasing pH, which is above the isoelectric point [49].

What is the isoelectric point of MBP?

The isoelectric point for MBP is 5.22, and hence the protein is negatively charged under physiological conditions.

What is the MBP tag protein purification?

Maltose binding protein (MBP) is a common protein expression tag, as it is known to docHubly enhance the solubility of many proteins. Fusion of a target protein to MBP permits one-step purification using amylose resin. The MBP-fusion protein will bind to amylose resin while other proteins flow through.

What is the isoelectric point of myelin basic protein?

MBP is extremely basic (isoelectric point: 10) and carries a highly positive charge [3].

What is the function of the maltose binding protein?

MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. The mechanism by which MBP increases solubility is not well understood.

What is the isoelectric point of protein solubility?

The isoelectric point is simply the pH at which the net charge of the amino acid chain of a protein is zero. More importantly, it coincides with the pH where the protein is least soluble and thus most likely to precipitate out of solution.

What is the isoelectric point of a protein pI?

The isoelectric point (pI) of a protein is defined as the pH at which the net charge of a protein molecule is zero. ingly, proteins are positively charged at a pH below their pI and negatively charged at a pH above their pI.

How to cleave MBP tag?

Following amylose purification, the target protein can be cleaved from the MBP-tag using TEV Protease, without adding any vector-derived residues to the protein. Both the MBP-tag and TEV Protease are polyhistidine-tagged for easy removal from the reaction.

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Inject identification in MBP

Inject identification in MBP with our multi-function editing solution

How to inject identification in MBP

PDF editing simplified with DocHub

Drive efficiency with the DocHub add-on for Google Workspace

How to inject identification in MBP

Got questions?

What is the isoelectric point of meat protein?

What is the isoelectric point of MBP?

What is the MBP tag protein purification?

What is the isoelectric point of myelin basic protein?

What is the function of the maltose binding protein?

What is the isoelectric point of protein solubility?

What is the isoelectric point of a protein pI?

How to cleave MBP tag?

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