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all equipment use in the procedure room is understood to be sanitized to current federal standards before opening your kit create a sterile working station peel back the outer seal and carefully remove the kit contents wipe the ceiling port of your anticoagulant with a sterile alcohol pad prior to inserting with a stealth syringe attach the nail to one of the 60 ml syringes before drawing the anticoagulant primed the syringe to ensure the barrel moves freely this is done by simply pulling back and forth on the plunger two to three times draw nine mlamp;#39;s of anticoagulant into the 60 ml syringe draw a whole blood from the patient filling the syringe to a maximum of 60 MLS once the blood is drawn detach the tube and ensure the anticoagulant mixes throughout the blood sample using the 45-degree dispensing tip slowly transfer the anticoagulated whole blood into the excel concentrating device secure the green silicon cap and the clear safety cap to the c