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why compare problems of course to study expression levels of normal versus disease cells that is for example to identify targets and device drug molecules and how mass spectrometry can be used to relatively quantify proteins what is the simplest procedure we can think of so what we can do is simply we take three solutions from a normal versus is disease and we do basically three bottom-up experiments for each of these samples and we obtain protein IDs as well as quantities and then basically do the statistics for each of the proteins that are there to get the normal vs. in disease and identify if there is a docHub change I want to mention here that one of the fundamental assumptions and how we go about doing this experiment is that we start with the exact same number of protein amounts in the total protein amounts that is based on the hypothesis that the total protein amount does not change docHubly for instance if you treat with a molecule or when there is a disease State h
