Fix sample in PAP

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Aug 6th, 2022
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How to fix sample in PAP

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- The cervix is the lower narrow end of the uterus that connects to the top of the vagina. The cervix is covered by a thin layer of tissue made up of cells. Cancer of the cervix, or cervical cancer, can develop when cells become abnormal. Most cases of cervical cancer are caused by infection with a virus called HPV, or human papillomavirus. The HPV virus can cause cells to become abnormal over time. One way to prevent HPV infection is by getting the HPV vaccine. You can also prevent cervical cancer by having regular screening tests and follow-up treatments if needed. Cervical cancer screening may include Pap tests, HPV tests, or both. Pap tests check for abnormal cells in your cervix. HPV tests check for the HPV virus. In both tests, cells are taken from the cervix in the same way and sent to a lab for testing. Cervical cancer screening is recommended starting at age 21. How often youamp;#39;re screened and which tests you have depends on your age and health history. It takes many yea

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Atypical repair is characterized by reparative cells that have cytologic abnormalities that exceed those of typical repair. Atypical repair is a form of atypical squamous or glandular cells associated with a wide variety of conditions ranging from reactive to neoplastic.
Out of which, 95% ethanol is the commonly used fixative. Hence, the conventional method for fixation of Pap smear is to fix the Pap smear immediately in 95% ethyl alcohol after preparing the smear.
Smears must be fixed immediately before they dry. A 95% solution of ethyl alcohol or spray fixative is used to fix the slide. The slides should be labeled in pencil with the patients name AND date of birth and a properly completed Pap smear requisition should accompany the slide.
Ethyl alcohol is the fixative specifically recommended for cytological preparations. Fixation coarsens the cell structures and sharpens nuclear chromatin pattern and its details.
Typically, your doctor will use the Loop Electrosurgical Excision Procedure (LEEP), or perform a cold knife cone biopsy as a minor surgical procedure. Be sure to always bring up any questions you may about you Pap test with your gynecologist.
The smear(s) should be immediately fixed with spray fixative or fixed in 95% ethyl alcohol for 10 minutes. Allow the slides to completely air dry before shipping. Scrape additional areas of the vagina that appear abnormal and spread and fix as noted above.
Unlike conventional Pap smears, LBC involves collecting samples, usually with a small brush, and suspending them in a liquid medium. This technique, based on monolayer slide preparation, offers improved specimen quality and has outperformed traditional methods.
Pap staining procedure Mayer Hematoxylin and Gills hematoxylin is preferred. In regressive method the smear is over stained with nuclear stain with a non-acidified hematoxylin. The excess stain is removed with dilute HCL acid solution. Harris hematoxylin is preferred for regressive method.

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