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[Music] hello everyone my name is Celia and Im an application specialist in vitam company in one of our previous videos we discussed chemical basics of one ghazan steam and today we will show you how to stay in slides on practice to stay in slides with one ghazan method we will need vigorous hematoxylin and we will prepare it as a mixture of solutions a and B right before staining and a mixture of picric acid and easy toxin depropanizer and rehydrated slides with samples we firstly put in distilled water then we use vagrant hematoxylin we need to prepare it right before staining as the mixture of solutions a and B we put slides in this mixture for 10 minutes then we put slides under the running water for 10 minutes then we use Regency which is a mixture of a sheet foxing and picric acid and put slide there for 5 minutes then we have to wash slides quickly in distilled water and then dehydrate them in alcohol clear and coverslip after that slides can be studied under the microscope to