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Record pressed, ready to go! Thank you for all the comments weve received over the last few weeks regarding our FlowJo series. And on those lines, weve had a lot of requests to do a tutorial on stain index. So thats what were looking at today. So thanks for joining and lets hop right in. So you can see in my workspace here, I have a series of different antibodies Ive titrated. So lets start with the B220. So if I open this up, youll see I already have a cells gate, which leads into my single cells gate, and then into my B220 populations. So on this plot here you can see we have a well-defined negative and a well-defined positive. So lets get some gates over top of those. So our negative gates. Negative. And our positive gate. Perfect. Now the next thing we need to do is pull out the median of our positive, the median of our negative, and our standard deviation. The easiest way to pull this out is using the table editor. So if we click on this, wel