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foreign here is an image of neuron staining and we will use neuron J plugin to perform neurite tracing and Analysis head to the website as shown in the screen below the link is also available in the description scroll down to find the neuron J and the image signs plugins download and save the plugins individually please once the download has been completed the plugins click on plugins the image signs dot jar plugin and save it in the plugins folder similarly the neuronj.jar plugin to find out if the plugins has been successfully installed click on plugins and find the neuronj plugin now open the image of Interest using image J since neuron J requires an 8-bit image therefore convert the RGB image into an 8-bit Type image adjust the brightness and contrast if required and then save this image in any format of your choice to use the neuronj plugin click on plugins and neuron J at this time the image J toolbar is replaced by the neuron J Toolbar to open the 8-Bit image click on th