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this is a short screencast to demonstrate a new mode of interaction which is available in the simple neurite tracer plugin for fiji iamp;#39;ve already loaded in here a nice data set this is an olfactory projection neuron from drosophila taken from the diadem challenge data sets kindly provided by greg jeffries and the way that one would normally trace within this data set using this plug-in or the the two recommended way is to find points within the two and a half d view this stack here and click to start a path you see a little blue square appear there move further along the path i click there and the plugin will search how to path between those two points if youamp;#39;re happy with that you would click yes to confirm you want to keep the path segment and then move further along the neuron in the same way and this works fine as you can see the results are reflected in the 3d viewer here but it wasnamp;#39;t up to this point possible to actually interact with the tracing mechanism