Fill in stain in PAP

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Aug 6th, 2022
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How to fill in stain in PAP

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hello and welcome to the channel love of status and gynecology in this video iamp;#39;ll explain about the technique of a pap smear and the liquid-based cytology for the cervical screening so the things that you will require for a pap smear or a liquid-based cytology a pair of gloves some clean glass slides this is known as the iron spatula the cyto brush we have the cuscoamp;#39;s by well speculum and for the liquid-based cytology we have this one known as the cytobroom and a liquid-based cytology while so beginning with the processor so first you need to perform an adequate hand hygiene and after that done a sterile pair of gloves as per your size and then finally you will introduce the cuscoamp;#39;s speculum see cusco speculum is for the retraction of anterior and the posterior vaginal wall right but we donamp;#39;t introduce it as such itamp;#39;s very uh very traumatic for the patient so basically you rotate it to a 90 degree like that and then you insert it right through an

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It is a progressive and differential stain that generally uses 5 dyes in 3 solutions. The color reaction depends on the type of dyes used in the Papanicolaou stain. It uses hematoxylin as nuclear stain, with 2 counterstains (orange G and Eosin Azure or EA). Papanicolaou Stain - an overview | ScienceDirect Topics sciencedirect.com medicine-and-dentistry sciencedirect.com medicine-and-dentistry
Wash slides in deionized or distilled water for 5 min at room temperature. Destain slides in 0.5% HCl in 70% ethanol for 5-15 min at room temperature. Wash slides in deionized or distilled water for 5 min at room temperature. Air dry sections.
Pap Staining involves five dyes in three solutions, a nuclear stain and two counter stains: Nuclear Stain: Harris Haematoxylin which is used to stain cell nuclei. Counter Stain OG6: Orange G dye is used to stain keratin. Its original role was to stain the small cells of keratinizing squamous cells present in sputum.
The first staining solution contains haematoxylin which stains cell nuclei. Papanicolaou used Harriss hematoxylin in all three formulations of the stain he published. The second staining solution (designated OG-6), contains Orange G in 95% ethyl alcohol with a small amount of phosphotungstic acid.
The filipin method uses cholesterol esterase and the fluorescent dye filipin to form a complex with cholesterol that fluoresces under UV light. It is used to label free cholesterol on biofilm structures and in non-cellular structures. Filipin - an overview | ScienceDirect Topics sciencedirect.com medicine-and-dentistry sciencedirect.com medicine-and-dentistry
Saline solution may be used for moistening. The lubricant does not dissolve during processing of the slide. It often stains dark purple and may hide numerous cells. Before scraping, the wooden spatula should be dipped in saline to moisten it and prevent too rapid dehydration of the scraped cells.

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